What Is TB-500?
TB-500 is a synthetic peptide fragment representing the active region of Thymosin Beta-4 (specifically the N-terminal acetylated tetrapeptide LKKTETQ motif). Thymosin Beta-4 is an endogenous protein responsible for actin filament sequestration, cell migration, endothelial cell differentiation, and dermal tissue regeneration.
In cell culture and animal models, TB-500 promotes vascular endothelial growth factor (VEGF) expression and tissue repair. It is routinely studied alongside BPC-157 or evaluated in pre-formulated co-lyophilized vials like the BPC-157 & TB-500 Blend. TB-500 features a circulating half-life of approximately 24 hours. Reconstituting TB-500 with our peptide calculator and bacteriostatic water ensures exact milligram dose accuracy.
In cell culture and animal models, TB-500 promotes vascular endothelial growth factor (VEGF) expression and tissue repair. It is routinely studied alongside BPC-157 or evaluated in pre-formulated co-lyophilized vials like the BPC-157 & TB-500 Blend. TB-500 features a circulating half-life of approximately 24 hours. Reconstituting TB-500 with our peptide calculator and bacteriostatic water ensures exact milligram dose accuracy.
TB-500 Dosage Chart
TB-500 research protocols frequently utilize milligram-scale doses (e.g. 2.0 mg or 2.5 mg per administration). Calculating concentrations accurately prevents excessive liquid injection volumes.
Vial: 2 mgWater: 1.0 mL
Concentration2.0 mg/mL
Target Dose2.0 mg (2,000 mcg)
Draw to Mark:100.0 Units
Vial: 5 mgWater: 2.0 mL
Concentration2.5 mg/mL
Target Dose2.5 mg (2,500 mcg)
Draw to Mark:100.0 Units
Vial: 10 mgWater: 2.0 mL
Concentration5.0 mg/mL
Target Dose2.5 mg (2,500 mcg)
Draw to Mark:50.0 Units
Vial: 20 mgWater: 4.0 mL
Concentration5.0 mg/mL
Target Dose5.0 mg (5,000 mcg)
Draw to Mark:100.0 Units
| Vial Size | Bac Water Added | Concentration | Target Research Dose | Draw Units (U-100) |
|---|---|---|---|---|
| 2 mg | 1.0 mL | 2.0 mg/mL | 2.0 mg (2,000 mcg) | 100.0 Units |
| 5 mg | 2.0 mL | 2.5 mg/mL | 2.5 mg (2,500 mcg) | 100.0 Units |
| 10 mg | 2.0 mL | 5.0 mg/mL | 2.5 mg (2,500 mcg) | 50.0 Units |
| 20 mg | 4.0 mL | 5.0 mg/mL | 5.0 mg (5,000 mcg) | 100.0 Units |
How to Reconstitute TB-500
Reconstituting TB-500 lyophilized powder requires standard laboratory diluent procedures to preserve peptide bioactivity.
Visual Standard Operating Procedure
TB-500 Step-by-Step SOP
5 Standard Reconstitution Steps for TB-500
- Wipe the rubber stoppers of both the TB-500 vial and bacteriostatic water vial with 70% isopropyl alcohol.
- Draw 2.0 mL of bacteriostatic water into a sterile syringe.
- Inject the diluent slowly down the interior glass wall of the TB-500 vial.
- Swirl the vial gently until the lyophilized powder dissolves completely. Avoid shaking.
- Refrigerate reconstituted solution at 2°C–8°C.
❄️ Storage & Shelf-Life Notice (TB-500)
TB-500 reconstituted with Bacteriostatic Water remains stable for up to 30 days under refrigeration (2°C–8°C). Keep away from light.
⚠️ Common Preparation Mistakes to Avoid
Injecting water under high pressure directly onto the lyophilized cake, shaking vigorously, and storing reconstituted liquid above 8°C.
TB-500 Dosing FAQ
How much bac water do I add to 10mg TB-500?↓
Adding 2.0 mL of bac water to a 10 mg TB-500 vial creates a concentration of 5.0 mg/mL (50 units = 2.5 mg).
How many units is 2.5 mg TB-500 on a 5mg vial?↓
If a 5 mg vial is mixed with 2.0 mL of water (2.5 mg/mL), a 2.5 mg dose is 100 units (1.0 mL).
What is the half-life of TB-500?↓
TB-500 has an elimination half-life of roughly 24 hours, supporting bi-weekly or weekly research dosing schedules.
Is TB-500 the same as Thymosin Beta-4?↓
TB-500 is a synthetic peptide fragment containing the active 4-amino-acid region of the naturally occurring Thymosin Beta-4 protein.
Why combine TB-500 with BPC-157?↓
BPC-157 promotes localized angiogenesis and mucosal repair, while TB-500 enhances systemic actin sequestration and cellular mobility.
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