What Is Mots-c?
MOTS-c (Mitochondrial Open Reading Frame of the 12S rRNA Type-c) is a 16-amino-acid peptide encoded within the mitochondrial genome. Functioning as a mitochondrial signal transducer, MOTS-c translocates to the cell nucleus during metabolic stress to regulate nuclear gene expression, activate 5′-AMP-activated protein kinase (AMPK), and enhance skeletal muscle insulin sensitivity.
MOTS-c is extensively studied in exercise mimetic research, metabolic longevity, and systemic lipid oxidation. It is frequently evaluated alongside metabolic research peptides like AOD-9604 and PT-141. MOTS-c has a circulating plasma half-life of approximately 4 hours. Reconstituting MOTS-c using our peptide calculator and bacteriostatic water guarantees precise milligram dosing.
MOTS-c is extensively studied in exercise mimetic research, metabolic longevity, and systemic lipid oxidation. It is frequently evaluated alongside metabolic research peptides like AOD-9604 and PT-141. MOTS-c has a circulating plasma half-life of approximately 4 hours. Reconstituting MOTS-c using our peptide calculator and bacteriostatic water guarantees precise milligram dosing.
Mots-c Dosage Chart & Peptide Dose Protocols
Unlike microgram-dosed peptides, MOTS-c research protocols involve higher milligram doses (typically 5.0 mg to 10.0 mg per trial session). Reconstituting a 10 mg vial with 2.0 mL of bacteriostatic water creates a concentration of 5.0 mg/mL, making a 5.0 mg dose equal 100 units (1.0 mL).
Vial: 5 mgWater: 1.0 mL
Concentration5.0 mg/mL
Target Dose5.0 mg (5,000 mcg)
Draw to Mark:100.0 Units (1.0 mL)
Vial: 10 mgWater: 2.0 mL
Concentration5.0 mg/mL
Target Dose5.0 mg (5,000 mcg)
Draw to Mark:100.0 Units (1.0 mL)
Vial: 10 mgWater: 1.0 mL
Concentration10.0 mg/mL
Target Dose5.0 mg (5,000 mcg)
Draw to Mark:50.0 Units
Vial: 20 mgWater: 2.0 mL
Concentration10.0 mg/mL
Target Dose10.0 mg (10,000 mcg)
Draw to Mark:100.0 Units
| Vial Size | Bac Water Added | Concentration | Target Research Dose | Draw Units (U-100) |
|---|---|---|---|---|
| 5 mg | 1.0 mL | 5.0 mg/mL | 5.0 mg (5,000 mcg) | 100.0 Units (1.0 mL) |
| 10 mg | 2.0 mL | 5.0 mg/mL | 5.0 mg (5,000 mcg) | 100.0 Units (1.0 mL) |
| 10 mg | 1.0 mL | 10.0 mg/mL | 5.0 mg (5,000 mcg) | 50.0 Units |
| 20 mg | 2.0 mL | 10.0 mg/mL | 10.0 mg (10,000 mcg) | 100.0 Units |
How to Reconstitute Mots-c
Reconstituting MOTS-c lyophilized powder requires care to prevent mitochondrial peptide cleavage.
Syringe Reading Diagram
U-100 Syringe Graduation & Measurement Line (Mots-c)
5 Standard Reconstitution Steps for Mots-c
- Sanitize the stoppers of both vials with 70% isopropyl alcohol prep pads.
- Draw 2.0 mL of sterile bacteriostatic water into a syringe.
- Inject water slowly down the interior glass wall of the MOTS-c vial.
- Swirl gently until completely clear. Do not shake.
- Refrigerate reconstituted solution at 2°C–8°C.
❄️ Storage & Shelf-Life Notice (Mots-c)
Reconstituted MOTS-c prepared with Bacteriostatic Water is stable for up to 28 days under refrigeration (2°C–8°C). Protect from direct light.
⚠️ Common Preparation Mistakes to Avoid
Shaking violently, using unpreserved water, and exposing reconstituted liquid to repeated freeze-thaw cycles.
Mots-c Dosing FAQ
How much bac water do I add to 10mg MOTS-c?↓
Adding 2.0 mL of bac water to a 10 mg MOTS-c vial creates a concentration of 5.0 mg/mL. A 5.0 mg dose equals 100 units (1.0 mL) on a U-100 syringe.
What is a mitochondrial-derived peptide?↓
Mitochondrial-derived peptides (MDPs) like MOTS-c are encoded directly within mitochondrial DNA rather than nuclear DNA, acting as metabolic signaling hormones.
How many units is 5 mg MOTS-c on a 10mg vial with 1mL water?↓
With 1.0 mL of water in a 10 mg vial (10 mg/mL concentration), a 5.0 mg dose equals 50 units (0.50 mL).
What is the half-life of MOTS-c?↓
MOTS-c has a circulating plasma half-life of roughly 4 hours, though its cellular AMPK activation effects last longer.
Can MOTS-c be frozen after reconstitution?↓
No. Reconstituted liquid peptide solutions must never be frozen. Store refrigerated at 2°C–8°C.