GHK-Cu Dosage Calculator

What Is GHK-Cu?

GHK-Cu (Glycyl-L-histidyl-L-lysine copper complex) is a naturally occurring human plasma copper-binding tripeptide. First isolated in 1973 by Dr. Loren Pickart, GHK-Cu regulates over 4,000 human genes, shifting gene expression toward tissue repair, collagen and elastin synthesis, antioxidant enzyme production (superoxide dismutase), and anti-inflammatory pathways.

GHK-Cu is distinct due to its high vial mass (typically 50 mg to 100 mg per vial) and intense deep blue solution color. It is studied standalone or in skin/repair stacks like the Klow Peptide Blend alongside anti-inflammatory peptides like KPV. Utilizing our peptide calculator with bacteriostatic water provides precise milligram concentration calculations for high-mass vials.

GHK-Cu Dosage Chart & Injection Dosage Protocols

Because GHK-Cu vials contain large quantities of active compound (50 mg or 100 mg), selecting the correct reconstitution volume is critical. Adding 3.0 mL of bacteriostatic water to a 50 mg vial yields a concentration of 16.67 mg/mL (1,667 mcg per 10 units), keeping daily injection volumes comfortable while maintaining accurate dosing ticks.

Vial: 20 mgWater: 2.0 mL

Concentration10.0 mg/mL

Target Dose1.0 mg (1,000 mcg)

Draw to Mark:10.0 Units

Vial: 50 mgWater: 3.0 mL

Concentration16.67 mg/mL

Target Dose2.0 mg (2,000 mcg)

Draw to Mark:12.0 Units

Vial: 50 mgWater: 5.0 mL

Concentration10.0 mg/mL

Target Dose2.0 mg (2,000 mcg)

Draw to Mark:20.0 Units

Vial: 100 mgWater: 5.0 mL

Concentration20.0 mg/mL

Target Dose2.0 mg (2,000 mcg)

Draw to Mark:10.0 Units

Vial Size	Bac Water Added	Concentration	Target Research Dose	Draw Units (U-100)
20 mg	2.0 mL	10.0 mg/mL	1.0 mg (1,000 mcg)	10.0 Units
50 mg	3.0 mL	16.67 mg/mL	2.0 mg (2,000 mcg)	12.0 Units
50 mg	5.0 mL	10.0 mg/mL	2.0 mg (2,000 mcg)	20.0 Units
100 mg	5.0 mL	20.0 mg/mL	2.0 mg (2,000 mcg)	10.0 Units

How to Reconstitute GHK-Cu

Reconstituting high-mass GHK-Cu vials requires adequate diluent volume to ensure complete dissolution without solution saturation.

Syringe Reading Diagram

U-100 Syringe Graduation & Measurement Line (GHK-Cu)

5 Standard Reconstitution Steps for GHK-Cu

Swab the stoppers of both the GHK-Cu vial and bacteriostatic water vial with 70% isopropyl alcohol.
Draw 3.0 mL or 5.0 mL of bacteriostatic water into a sterile syringe.
Inject water slowly against the glass wall of the GHK-Cu vial.
Swirl gently until the dark blue powder dissolves into a crystal-clear deep blue liquid solution.
Store reconstituted GHK-Cu in a refrigerator at 2°C–8°C.

❄️ Storage & Shelf-Life Notice (GHK-Cu)

Reconstituted GHK-Cu solution is deep blue in appearance. When reconstituted with 0.9% benzyl alcohol bacteriostatic water, it remains stable under refrigeration (2°C–8°C) for up to 30 days.

⚠️ Common Preparation Mistakes to Avoid

Mistaking the intense blue color for chemical degradation, adding too little water (leading to localized sting upon research administration), and shaking violently.

GHK-Cu Dosing FAQ

Why is reconstituted GHK-Cu solution deep blue?↓

GHK-Cu contains a chelated copper (II) ion bound to the tripeptide chain, which naturally imparts a vibrant blue color in aqueous solution.

How much bac water for a 50mg GHK-Cu vial?↓

Adding 3.0 mL of bac water to a 50 mg vial creates a concentration of 16.67 mg/mL (12 units = 2.0 mg). Adding 5.0 mL creates a concentration of 10 mg/mL (20 units = 2.0 mg).

How many units is 2 mg GHK-Cu on a 50mg vial with 3mL water?↓

With 3.0 mL of water added to 50 mg GHK-Cu, a 2.0 mg dose equals 12 units (0.12 mL) on a U-100 syringe.

Does GHK-Cu cause post-injection sting?↓

In laboratory studies, highly concentrated copper peptide solutions can cause localized tissue sensation. Increasing reconstitution volume (e.g. 5.0 mL water) reduces osmolarity.

How does GHK-Cu differ from non-copper GHK?↓

GHK-Cu includes the chelated copper ion required for vascular and enzymatic collagen synthesis, whereas GHK lacks the copper cofactor.