AOD-9604 Dosage Calculator

Last updated: 2026-11-10

Sources checked: 2026-11-10

📌At a Glance: AOD-9604 Reconstitution Arithmetic

Growth Hormone Fragment, Not Somatropin: AOD-9604 is a synthetic 16-amino-acid C-terminal fragment of human growth hormone (Tyr-hGH 177–191). It is measured strictly in milligrams (mg) or micrograms (mcg), never in somatropin International Units (IU).
Never Apply IU Conversions: Recombinant human growth hormone products use a standard calibration of approximately 1 mg = 3 IU. This conversion factor does NOT apply to AOD-9604; attempting to convert AOD-9604 to IU creates severe dosage calculation errors.
Common Research Vial Masses: Standard laboratory lyophilized powder cakes contain 2 mg, 5 mg, and 10 mg of active peptide substance.
Syringe Calibration: All volumetric conversions assume a standard U-100 insulin syringe (100 units = 1.0 mL, or 0.01 mL per unit graduation mark).

⚖️Regulatory & Compound Status

Verified: 2026-11-10

AOD-9604 is not an FDA-approved drug and has not been cleared for human clinical therapy or prescription treatment. The FDA has placed AOD-9604 on the Category 2 bulk drug substances list under Section 503A of the FD&C Act, citing significant safety and immunogenicity concerns that restrict legal compounding. The World Anti-Doping Agency (WADA) explicitly prohibits AOD-9604 under Section S2 (Peptide Hormones, Growth Factors, and Related Substances) at all times. Published clinical studies evaluate systemic tolerability in healthy human volunteers without IGF-1 elevation (Journal of Endocrinology and Metabolism 2014). Lyophilized vials sold online are unapproved research chemicals.

Official Sources:Safety and Tolerability of the Hexadecapeptide AOD9604 in Humans,World Anti-Doping Code International Standard: Prohibited List (S2: Peptide Hormones),Safety Risks Associated with Certain Bulk Drug Substances Nominated for 503A/503B Compounding

ℹ️What This Tool Cannot Tell You

This calculator cannot provide medical advice, diagnosis, or clinical administration protocols.
It cannot provide metabolic recommendations or therapeutic treatment plans.
It cannot verify the chemical purity, sterility, or peptide content of unapproved research chemicals.
It cannot guarantee solution stability beyond documented USP <797> multi-dose preservative limits.

This tool converts volumetric liquid measurements and syringe tick marks only. It does not provide medical guidance, dosing schedules, or clinical administration protocols. Review our Medical & Research Disclaimer.

What Is AOD-9604 Reconstitution Arithmetic?

AOD-9604 (Advanced Obesity Drug 9604) is a synthetic modified peptide fragment corresponding to the lipolytic C-terminal region of human growth hormone (hGH residues 177–191 with an added N-terminal tyrosine residue: Tyr-Leu-Arg-Ile-Val-Gln-Cys-Arg-Ser-Val-Glu-Gly-Ser-Cys-Gly-Phe). The peptide features an internal cyclic disulfide bridge between Cys182 and Cys189. Preclinical and early clinical evaluations (PubMed PMID: 24707348) investigated its capacity to stimulate beta-3 adrenergic pathways in adipocytes without binding to classic growth hormone somatogenic receptors or altering serum IGF-1 concentrations.

Analytical research facilities acquire AOD-9604 as a freeze-dried, solid-cake lyophilized powder in glass serum vials. Reconstitution is the chemical dissolution of this cake in a sterile diluent, primarily bacteriostatic water (sterile water for injection containing 0.9% benzyl alcohol). Our aod 9604 dosage calculator computes exact solution concentrations and translates microgram test amounts into syringe graduation marks without mathematical errors.

AOD-9604 Dosage Chart

This reference chart translates intended microgram test amounts into volumetric draw units on a standard U-100 syringe across 2 mg, 5 mg, and 10 mg research vials. These figures represent illustrative mathematical conversions across uniform benchmark test amounts (250 mcg, 300 mcg, 500 mcg, 1,000 mcg) and do not represent medical advice, clinical protocols, or therapeutic recommendations.

Vial Size	Bac Water Added	Concentration	Example Amount	Draw Units (U-100)
2 mg	1.0 mL	2,000 mcg/mL	250 mcg	12.5 Units
2 mg	2.0 mL	1,000 mcg/mL	300 mcg	30.0 Units
5 mg	2.0 mL	2,500 mcg/mL	250 mcg	10.0 Units
5 mg	2.0 mL	2,500 mcg/mL	300 mcg	12.0 Units
5 mg	2.0 mL	2,500 mcg/mL	500 mcg	20.0 Units
10 mg	2.0 mL	5,000 mcg/mL	300 mcg	6.0 Units
10 mg	2.0 mL	5,000 mcg/mL	500 mcg	10.0 Units
10 mg	2.0 mL	5,000 mcg/mL	1,000 mcg (1.0 mg)	20.0 Units

AOD-9604 Vial Size and Diluent Conversion Matrix

The table below outlines resulting solution concentrations, unit potencies, and draw units required per 1.0 mg across standard research vials (2 mg, 5 mg, 10 mg) and diluent volumes (1 mL, 2 mL, 3 mL). All values follow the deterministic formula: Concentration (mg/mL) = Vial Mass (mg) ÷ Diluent Volume (mL).

Vial Mass (mg)	Diluent Added (mL)	Concentration (mg/mL)	Potency per Unit (mcg/U)	Units per 1.0 mg Draw
2 mg	1.0 mL	2.00 mg/mL	20.0 mcg/U	50.0 Units
2 mg	2.0 mL	1.00 mg/mL	10.0 mcg/U	100.0 Units
2 mg	3.0 mL	0.67 mg/mL	6.7 mcg/U	150.0 Units
5 mg	1.0 mL	5.00 mg/mL	50.0 mcg/U	20.0 Units
5 mg	2.0 mL	2.50 mg/mL	25.0 mcg/U	40.0 Units
5 mg	3.0 mL	1.67 mg/mL	16.7 mcg/U	60.0 Units
10 mg	1.0 mL	10.00 mg/mL	100.0 mcg/U	10.0 Units
10 mg	2.0 mL	5.00 mg/mL	50.0 mcg/U	20.0 Units
10 mg	3.0 mL	3.33 mg/mL	33.3 mcg/U	30.0 Units

Worked Calculation Example: 5 mg Vial with 2.0 mL Diluent

Example Parameters:

Vial Mass: 5.0 mg solid lyophilized cake (5,000 mcg)
Diluent Added: 2.0 mL sterile Bacteriostatic Water
Target Test Amount: 500 mcg (0.5 mg) illustrative calculation
Syringe Standard: U-100 insulin syringe (100 units = 1.0 mL, or 0.01 mL per unit)

Step-by-Step Calculation:

Step 1: Calculate solution concentration = 5.0 mg ÷ 2.0 mL = 2.5 mg/mL (2,500 mcg/mL).
Step 2: Determine volumetric draw = 500 mcg ÷ 2,500 mcg/mL = 0.20 mL.
Step 3: Convert volume to U-100 syringe units = 0.20 mL × 100 units/mL = 20.0 Units.
Step 4: Mathematical Back-Check: 20.0 units × 0.01 mL/unit = 0.20 mL. 0.20 mL × 2.5 mg/mL = 0.50 mg = 500 mcg. Result confirms target mass exactly.

Result:

Drawing 20.0 units on a U-100 syringe delivers exactly 500 mcg of AOD-9604.

Note: For an illustrative 300 mcg test amount from this same vial: 300 mcg ÷ 2,500 mcg/mL = 0.12 mL = 12.0 units.

Growth Hormone Fragment Math: Milligrams vs. International Units (IU)

The single most common conceptual error regarding AOD-9604 is attempting to calibrate it using growth hormone International Units (IU):

1. The Recombinant hGH Standard: In pharmaceutical medicine, recombinant human growth hormone (somatropin) is calibrated against an international biological standard established by the World Health Organization, where 1 milligram of somatropin equals approximately 3.0 International Units (IU) (or 1 IU = 0.33 mg). Somatropin is a full-length 191-amino-acid protein (MW ~22,124 Da) with specific receptor-binding bioactivity.

2. Why IU Conversions Do NOT Apply to AOD-9604:
• AOD-9604 is an isolated 16-amino-acid synthetic peptide fragment (MW ~1,815.1 g/mol). It does not activate systemic growth hormone receptors and possesses no established International Unit potency standard.
• Labeling or calculating AOD-9604 in 'IU' is chemically invalid. AOD-9604 is measured strictly in mass units: milligrams (mg) and micrograms (mcg).
• Applying somatropin's 3:1 conversion factor to AOD-9604 creates severe dosage errors. Always calculate AOD-9604 strictly using standard mass-to-volume dilution formulas.

For a step-by-step primer on handling decimal conversions, consult our tutorial on how to calculate peptide dosage.

Syringe Unit Resolution and Measurement Impact (for 300 mcg Target Draw)

This table illustrates what percentage of an illustrative 300 mcg target draw is contained in a single syringe unit mark on a standard U-100 syringe across various reconstitution setups.

Vial Setup	Concentration	Peptide Mass per Unit	One Unit as % of 300 mcg Target	Practical Measurement Note
5 mg in 2.0 mL	2,500 mcg/mL	25.0 mcg	8.33%	12.0 units = 300 mcg; clean graduation mark
5 mg in 3.0 mL	1,667 mcg/mL	16.7 mcg	5.56%	18.0 units = 300 mcg; expanded draw volume
2 mg in 2.0 mL	1,000 mcg/mL	10.0 mcg	3.33%	30.0 units = 300 mcg; high visual readability
10 mg in 2.0 mL	5,000 mcg/mL	50.0 mcg	16.67%	6.0 units = 300 mcg; 1 unit error = 16.7% deviation

AOD-9604 Chemical Stability & Laboratory Handling

AOD-9604 contains an internal cyclic disulfide loop between cysteine residues at positions 182 and 189 that preserves its secondary conformation. Premature agitation or high fluid shear during reconstitution can destabilize this loop and cause peptide precipitation. When reconstituting, inject bacteriostatic water slowly down the interior glass wall so the liquid flows down smoothly. Swirl the vial in slow circular motions until completely dissolved into a clear liquid. Do not shake vigorously.

Store reconstituted solutions upright in continuous refrigeration between 2°C and 8°C (USP <659>). Under FDA multi-dose sterile vial guidance and USP <797> standards, multi-dose vials preserved with 0.9% benzyl alcohol should be discarded within 28 days of first puncture unless product labeling specifies a shorter limit. Never freeze liquid reconstituted peptide solutions. For complete standard operating procedures, consult our guide on how to reconstitute peptides safely.

Common Calculation & Measurement Mistakes

⚠️Applying Somatropin (Growth Hormone) IU Conversions

AOD-9604 is an isolated 16-amino-acid synthetic peptide fragment, not full-length somatropin. It possesses no WHO biological International Unit standard. Calculating AOD-9604 using somatropin IU formulas causes severe mathematical errors.

⚠️Confusing Micrograms (mcg) with Milligrams (mg)

Entering 300 in a milligram field instead of a microgram field causes a 1,000× calculation error. A 300 mcg draw from a 5 mg/2 mL vial is 12 units (0.12 mL); 300 mg from that same vial would require 120 mL of liquid.

⚠️Altering Diluent Volume Without Recalculating

If you reconstitute a 5 mg vial with 1.0 mL of water (5 mg/mL) rather than 2.0 mL (2.5 mg/mL), the solution is twice as concentrated. Drawing the same syringe unit mark delivers twice the active peptide mass.

⚠️Using a U-40 Syringe for U-100 Volume Calculations

U-100 syringes contain 100 units per mL (0.01 mL/unit). U-40 veterinary syringes contain 40 units per mL (0.025 mL/unit). Drawing 20 units on a U-40 syringe delivers 0.50 mL instead of 0.20 mL—causing an unintentional 2.5× volumetric error.

Comparing AOD-9604 with Related Research Peptides

AOD-9604 is frequently evaluated alongside other metabolic and growth factor peptides. Understanding how its reconstitution arithmetic compares to related compounds prevents laboratory errors:

• Compared to MOTS-c: Both compounds are studied in metabolic research. However, MOTS-c vials are distributed in larger milligram masses (10 mg to 40 mg), requiring larger diluent volumes, whereas AOD-9604 vials typically contain 2 mg to 5 mg.
• Compared to CJC-1295 & Ipamorelin Blend: CJC-1295 and ipamorelin stimulate pituitary growth hormone pulsatility through GHRH and ghrelin receptors. In contrast, AOD-9604 is a direct fragment of the growth hormone molecule itself.
• Compared to Sermorelin: Sermorelin is a 29-amino-acid GHRH secretagogue evaluated in 3 mg and 6 mg vials. Both compounds are labeled in milligrams and follow identical mass-to-volume dilution formulas.

Reconstitution Protocol Summary

Lyophilized AOD-9604 cake dissolves cleanly when sterile diluent is introduced gently under aseptic conditions.

Syringe Reading Diagram

U-100 Syringe Graduation & Measurement Line (AOD-9604)

Standard Reconstitution Protocol

Review our standardized 5-step sterile reconstitution guide for full vial preparation, alcohol disinfection, and needle safety protocols.

Universal Step-by-Step Reconstitution Guide→

❄️ Storage & Shelf-Life Notice (AOD-9604)

Store reconstituted AOD-9604 solutions refrigerated at 2°C–8°C (36°F–46°F). Protect from prolonged ultraviolet light exposure. Never freeze reconstituted liquid solutions.

⚠️ Common Preparation Mistakes to Avoid

Common errors include applying somatropin IU conversions, entering micrograms into milligram fields, and using U-40 veterinary syringes.

AOD-9604 Dosing FAQ

Is AOD-9604 measured in International Units (IU)?↓

No. Full-length human growth hormone (somatropin) is calibrated in International Units (approximately 1 mg = 3 IU). AOD-9604 is an isolated 16-amino-acid synthetic fragment and is measured strictly in milligrams (mg) and micrograms (mcg). It has no valid IU conversion.

Is AOD-9604 the same molecule as human growth hormone (hGH)?↓

No. Human growth hormone is a full 191-amino-acid protein. AOD-9604 is a synthetic peptide fragment corresponding only to residues 177–191 with an added tyrosine residue. It lacks the functional domains that bind to systemic growth hormone receptors or stimulate IGF-1 release.

How do I convert milligrams to syringe units for AOD-9604?↓

Divide your target dose by your solution concentration to find volume in milliliters: Volume (mL) = Target Dose (mg) ÷ Concentration (mg/mL). Then multiply by 100 to determine U-100 syringe units. For example, 0.5 mg divided by 2.5 mg/mL equals 0.20 mL (20 units).

What syringe standard should be used for measuring AOD-9604 draws?↓

Calculations on this page are calibrated for standard U-100 insulin syringes (100 units = 1.0 mL, or 0.01 mL per unit). For microgram draws of 10 to 30 units (0.10 mL to 0.30 mL), a 0.3 mL or 0.5 mL U-100 syringe provides optimal visual line alignment.

Can reconstituted AOD-9604 liquid be frozen?↓

No. Liquid reconstituted peptide solutions must never be frozen. Freezing creates ice crystal lattices that can shear peptide chains and precipitate out of solution. Store liquid reconstituted vials refrigerated at 2°C–8°C.

Related Peptide Calculators & Stacks

CJC-1295 & Ipamorelin

Calculate CJC-1295 + Ipamorelin stack reconstitution, GHRH/GHRP secretagogue math, and syringe units.

Calculate CJC-1295 & Ipamorelin→

MOTS-c

Calculate reconstitution volume, solution concentration, and U-100 syringe units for research MOTS-c vials.

Calculate MOTS-c→

Sermorelin

Calculate Sermorelin GHRH analogue reconstitution, microgram dosing, and U-100 syringe units.

Calculate Sermorelin→

Related Reconstitution & Dosing Guides

View All FAQs→

MOTS-c FAQ

How to Reconstitute MOTS-c?

→

General Peptide FAQ

How to Reconstitute Peptides?

→